matpiv v. 1.6.1 Search Results


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Loss of actomyosin regulators results in reduced cytoplasmic streaming. a Representative images of the average velocity vectors <t>(PIV</t> analysis) of the cytoplasmic streaming in the distal region of control, cyk-1(or596) at 20 °C, nmy-2 (RNAi) on L4 stage worms, cyk-1 (RNAi) on cyk-1(or596) , nmy-2 (RNAi) on L3 stage worms, and mel-11 (RNAi) gonads. Images are oriented in the direction of distal (D) to proximal axis (P) of the gonad. b , c Average speed and average velocity of the cytoplasmic streaming (along the X -axis) in the distal region of control ( N = 17), cyk-1(or596) at 20 °C ( N = 22), nmy-2 (RNAi) on L4 stage worms ( N = 10), cyk-1 (RNAi) on cyk-1(or596) ( N = 11), nmy-2 (RNAi) on L3 stage worms ( N = 10), and mel-11 (RNAi) gonads ( N = 19). <t>The</t> <t>particle</t> speed and velocity was analyzed for 200 s in each movie. Data represents mean ± s.d. of four independent experiments. Statistical analysis was done using one-way ANOVA test (* p < 0.05, ** p < 0.005, *** p < 0.0001, ns, non-significant), N = number of gonads analyzed. Scale bar, 5 µm
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Loss of actomyosin regulators results in reduced cytoplasmic streaming. a Representative images of the average velocity vectors (PIV analysis) of the cytoplasmic streaming in the distal region of control, cyk-1(or596) at 20 °C, nmy-2 (RNAi) on L4 stage worms, cyk-1 (RNAi) on cyk-1(or596) , nmy-2 (RNAi) on L3 stage worms, and mel-11 (RNAi) gonads. Images are oriented in the direction of distal (D) to proximal axis (P) of the gonad. b , c Average speed and average velocity of the cytoplasmic streaming (along the X -axis) in the distal region of control ( N = 17), cyk-1(or596) at 20 °C ( N = 22), nmy-2 (RNAi) on L4 stage worms ( N = 10), cyk-1 (RNAi) on cyk-1(or596) ( N = 11), nmy-2 (RNAi) on L3 stage worms ( N = 10), and mel-11 (RNAi) gonads ( N = 19). The particle speed and velocity was analyzed for 200 s in each movie. Data represents mean ± s.d. of four independent experiments. Statistical analysis was done using one-way ANOVA test (* p < 0.05, ** p < 0.005, *** p < 0.0001, ns, non-significant), N = number of gonads analyzed. Scale bar, 5 µm

Journal: Nature Communications

Article Title: Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset

doi: 10.1038/s41467-018-07149-2

Figure Lengend Snippet: Loss of actomyosin regulators results in reduced cytoplasmic streaming. a Representative images of the average velocity vectors (PIV analysis) of the cytoplasmic streaming in the distal region of control, cyk-1(or596) at 20 °C, nmy-2 (RNAi) on L4 stage worms, cyk-1 (RNAi) on cyk-1(or596) , nmy-2 (RNAi) on L3 stage worms, and mel-11 (RNAi) gonads. Images are oriented in the direction of distal (D) to proximal axis (P) of the gonad. b , c Average speed and average velocity of the cytoplasmic streaming (along the X -axis) in the distal region of control ( N = 17), cyk-1(or596) at 20 °C ( N = 22), nmy-2 (RNAi) on L4 stage worms ( N = 10), cyk-1 (RNAi) on cyk-1(or596) ( N = 11), nmy-2 (RNAi) on L3 stage worms ( N = 10), and mel-11 (RNAi) gonads ( N = 19). The particle speed and velocity was analyzed for 200 s in each movie. Data represents mean ± s.d. of four independent experiments. Statistical analysis was done using one-way ANOVA test (* p < 0.05, ** p < 0.005, *** p < 0.0001, ns, non-significant), N = number of gonads analyzed. Scale bar, 5 µm

Article Snippet: As for v p , particle image velocimetry (PIV) analysis was performed using a MATLAB PIV toolbox, MatPIV 1.6.1 ( http://www.mn.uio.no/math/english/people/aca/jks/matpiv/ ).

Techniques: Control